Kerafast抗體Anti-DNA-RNA Hybrid [S9.6] Antibody 

產品介紹：

Kerafast的(de)貨號：ENH001，Anti-DNA-RNA Hybrid [S9.6] Antibody，這種小鼠單(dan)克隆抗體(ti)是針對 ΦX174 噬菌(jun)體(ti)衍(yan)生(sheng)的(de)合成 DNA-RNA 抗原生(sheng)成的(de)，可識別各種長度的(de) RNA-DNA 雜合體(ti)。

特色：

可用于檢測 R 環

對 DNA-RNA 雜交體的高特異性和親和力

不與單鏈 DNA 或雙鏈 DNA 發生交叉反應

對于(yu)富含 AU 的雙鏈(lian) RNA，觀察(cha)到了(le)輕(qing)微的交叉反應（約 5 倍以下）。

長(chang)度(du)為 8、10、15 和 23 個(ge)堿基對的雜交體顯示出高(gao)親和力(li)結合

DNA-RNA 雜(za)(za)合(he)體(ti)(ti)(ti)是真核細胞(bao)中的(de)一種自然現象，這些(xie)雜(za)(za)合(he)體(ti)(ti)(ti)的(de)水平在具有高轉(zhuan)錄活性的(de)位點增加，例如在轉(zhuan)錄起始、抑制和(he)延伸期間。由于 RNA-DNA 雜(za)(za)合(he)體(ti)(ti)(ti)會影響基因組的(de)不穩定性，因此 S9.6 抗體(ti)(ti)(ti)是一種有用的(de)試劑，可幫助(zhu)研(yan)究在 DNA 復制或其(qi)他細胞(bao)過程中由這些(xie)雜(za)(za)合(he)體(ti)(ti)(ti)形成的(de) R 環和(he)損傷的(de)后果(guo)。此外，S9.6 抗體(ti)(ti)(ti)可有效識(shi)別用于微陣列研(yan)究的(de) RNA-DNA 雜(za)(za)交。

This mouse monoclonal antibody was generated against a ΦX174 bacteriophage-derived synthetic DNA–RNA antigen and recognizes RNA-DNA hybrids of various lengths.

Highlights:

* Useful in the detection of R-loops

* High specificity and affinity for DNA-RNA hybrids

* Does NOT cross-react with single-stranded DNA or double-stranded DNA

* Minor cross-reaction (~5-fold less) has been observed for AU-rich double-stranded RNA.

* High affinity binding shown for hybrids of 8, 10, 15, and 23 base pairs in length

產品(pin)詳情(qing)：

Product Type:	Antibody
Name:	Anti-DNA-RNA Hybrid [S9.6]
Antigen:	S9.6 ΦX174 bacteriophage-derived synthetic DNA–RNA antigen
Isotype:	Rabbit IgG
Fusion Tag(s):	Mouse Fab version contains His-tag
Clone Name:	S9.6
Reactivity:	High specificity and affinity for DNA/RNA hybrids and other A-form nucleic acid hybrids
Immunogen:	ΦX174 bacteriophage-derived synthetic DNA/RNA
Purification Method:	Protein A/G
Buffer:	ENHOO1: PBS, 0.05% (w/v) Sodium Azide            Ab01137- : PBS with 0.02% Proclin 300
Tested Applications:	Dot Blot Analysis: 0.2 µg/mL.            Affinity Binding Assay: Clone S9.6 bound the DNA-RNA heteropolymer and poly(I)-poly(dC) equally, but 100-fold higher levels of poly(A)-poly(dT) were required to achieve a similar degree of binding. Single-stranded DNA, double-stranded DNA and RNA, and ribosomal RNA were not bound by clone S9.6 (Boguslawski, S.J., et al. (1986). J. Immunol Methods. 89(1):123-130).            Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected increased DNA RNA hybrids at four actively transcribed genes upon shRNA-mediated knockdown of BRCA1 or BRCA2, but not PCID2 or RAD51 in HeLa cells (Bhatia, V., et al. (2014). Nature. 511(7509):362-365).            Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected R-loops formed over beta-actin gene using HeLa chromatin preparation. RNase H treatment of the chromatin preparation prevented clone S9.6 from immunoprecipitating target chromatin fragments (Skourti-Stathaki, K., et al. (2011). Mol. Cell. 42(6):794-805).            Chromatin Immunoprecipitation-sequencing (ChIP-seq) Analysis: A representative lot detected genome-wide distribution of DNA-RNA hybrids in budding yeast by ChIP-seq analysis (El Hage, A., et al. (2014). PLoS Genet. 10(10):e1004716).            Immunocytochemistry Analysis: Representative lots immunolocalized nuclear R loops by fluorescent immunocytochemistry staining of methanol-fixed H1 human embryonic stem cells (hESCs) and formaldehyde-fixed HeLa cells (Bhatia, V., et al. (2014). Nature. 511(7509):362-365; Ginno, P.A., et al. (2012). Mol. Cell. 45(6):814-825).            Immunoprecipitation Analysis: A representative lot immunoprecipitated in vitro transcribed R-loop substrate (DNA-RNA hybrid), but not doouble-stranded DNA (dsDNA) (Ginno, P.A., et al. (2012). Mol. Cell. 45(6):814-825).

Kerafast抗體Anti-DNA-RNA Hybrid [S9.6] Antibody 文(wen)獻

參考文獻：

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